Antioxidant Activity of Aceriphyllum rossi Leaf Extracts using Different Solvents

자료유형:  학술저널

저자정보: 이재희, 이재형, 이기연, 장은하, 서영호, 김기선

저널정보: 강원대학교 농업생명과학연구원(구 농업과학연구소) >  농업생명환경연구 >  농업생명환경연구 제36권 제4호

KCI등재

발행연도: 2024.12

수록면: 375 - 386 (12page)

DOI: https://doi.org/10.22698/jales.20240028

ABSTRACT

The aim of this study was to evaluate the antioxidant potential of Aceriphyllum rossii leaf (ARL) extracts obtained using water and 10%, 30%, 50%, and 70% ethanol solutions as extraction solvents. These different extracts obtained from ARL, traditionally recognized for its health benefits, were analyzed to determine the effect of different solvent concentrations on the antioxidant extraction efficiency and antioxidant activities of these extracts. Antioxidant assays, including the DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS (2,2’-azinobis-[3-ethylbenzothiazoline-6-sulfonic acid]) radical scavenging assays and ORAC (oxygen radical absorbance capacity) and FRAP (ferric-reducing antioxidant power) assays, were performed to quantify the free radical scavenging capacity of each extract, alongside the measurements of total polyphenol and flavonoid contents. The results revealed that ethanol extracts consistently exhibited higher antioxidant activities and polyphenol contents than those of water extracts, with the 50% ethanol extract achieving the highest total polyphenol (381.27 mg/g) and flavonoid contents (176.53mg/g). Both 30% and 50% ethanol extracts demonstrated superior free radical scavenging activities, suggesting mid-range ethanol concentrations as optimal for efficiently extracting antioxidant compounds from ARL. Notably, the 30% ethanol extract maintained high antioxidant efficacy while using a lower ethanol concentration, which provides a cost-effective solution for industrial applications by reducing ethanol recovery and solvent costs. Therefore, the 30% ethanol

extract presents a practical choice for large-scale production, with potential applications as a natural antioxidant in functional foods and cosmetics. These findings highlight the importance of ARL as a promising source of antioxidants, emphasizing that optimal solvent selection can enhance both economic feasibility and functional benefits. Future studies are recommended to explore the in vivo efficacy and safety of ethanol-based ARL extracts, aiming to expand their potential as a versatile ingredient in health-promoting products.

Keywords: ABTS, Aceriphyllum rossii, Antioxidant activity, DPPH, Extraction solvent