자색옥수수 포엽 및 속대추출물의 Chinese Hamster Lung 세포를 이용한 염색체 이상시험

Genotoxicity Evaluation of Extracts from the Husks and Cobs of Purple Corn using an In vitro Chromosome Aberration Test in Chinese Hamster Lung Cells

농업생명환경연구

2021, vol.33, no.3, pp. 339-346 (8 pages)

DOI : 10.22698/jales.20210033

발행기관 : 강원대학교 농업생명과학연구원

연구분야 : 농수해양학 > 농학 > 농학일반 > 농업교육

초록

This study evaluated the toxicity of extracts from the husks and cobs of purple corn (HCPC) using an in vitro chromosomal aberration test in Chinese hamster lung (CHL) cells. Experimental groups were treated with –S9 mix (6 h), +S9 mix (6h), or –S9 mix (24 h). A dose range finding study was conducted by setting the concentrations of HCPC extracts to 156, 313, 625, 1,250, 2,500 and 5,000 μg/mL. Consequently, the –S9 mix (6h) RICC55 value was 1677.76 μg/mL, no cytotoxicity was determined at +S9 mix (6h), and –S9 mix (24h) RICC55 value was 582.91 μg/mL. Based on the results, concentration of experimental groups was determined by setting 1,000, 1,250, 1,500, and 1,750 μg/mL in –S9 mix (6 h); 1,250, 2,500, and 5,000 μg/mL in +S9 mix; and 100, 200, 400, 600, and 800 μg/mL in –S9 mix (24 h). The frequency of appearance of chromosomal dysplasia cells and numerical dysplasia cells was less than 5% each. In the positive controls, the frequency of appearance of structural dysplasia cells was more than 10%. Under the conditions of this study, extracts from the husks and cobs did not cause chromosome abnormalities in CHL cells.

키워드

Chromosome aberration test, Cobs, Husk, Purple corn