국가표준식품성분표 DB 구축을 위한 국내 농산물의 당류 성분 분석

Analyzing the Sugar Components of Domestic Agricultural Products for Establishing the National Standard Food Composition Data-Base

동아시아식생활학회지

2023, vol.33, no.1, pp. 71-80 (10 pages)

발행기관 : 동아시아식생활학회

연구분야 : 자연과학 > 생활과학

ABSTRACT

This study analyzes the sugar components of domestic agricultural products to establish a national standard food composition data-base. Seven sugars were simultaneously analyzed through HPLC-ELSD. The linearity, detection limit, and quantification limit of the standard solutions were calculated for verifying the analysis method and data quality control. The R2 (coefficient of determination) of all seven sugar standards was above 0.999, showing good linearity. The LOD (limit of detection) and LOQ (limit of quantification) ranged between 0.026∼0.198 mg/g and 0.073∼0.601 mg/g, respectively. CRM (Certified Reference Material) analysis was performed to determine the quality control of data analysis. The recovery ratio for the true values of fructose, glucose, sucrose, and lactose was calculated and confirmed to be in the range 94.50∼99.03%. In addition, results from FAPAS (Food Analysis Performance Assessment Scheme), confirmed that the z-scores of sucrose and glucose were within ± 1, thereby verifying the reliability of sugar analysis. Raw agricultural materials and simple processed agricultural products consumed domestically were then subjected to sugar analysis. Samples for sugar analysis were classified into light-leaved vegetables, fruits and vegetables, root vegetables, and pulses. The primary sugars obtained in the samples were fructose, glucose, and sucrose.

Key words: sugars, HPLC-ELSD, analysis, data base, agricultural products